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IL2 Protein (Native, Human)

CAT: 1543-P520527Size: 100 µgDry Ice: NoHazardous: No
CAT#:1543-P520527Size:100 µg
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Abbreviation
IL2
Synonyms
IL-2, lymphokine, TCGF
Host
E. coli
Tag
Native
Endotoxin
< 5 EU per mg of the protein.
Purity
≥ 95 % as determined by SDS-PAGE.≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Measured in a cell proliferation assay using CTLL2. The ED50 for this effect is typically 1-8 ng/mL. 2.Loaded Recombinant Rhesus IL-2R beta/CD122 Protein, His & AVI Tag, Biotinylated (Cat. No. 90328-K49H-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein, no Tag (Cat. No. 11848-HNAE2) with an affinity constant of 0.508 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human IL-2R beta / CD122 Protein, His Tag (Cat. No. 10696-H08H) on Ni-NTA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 0.313 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Human IL-2R beta/CD122 Protein, His Tag, Biotinylated, (Cat. No. 10696-H27H-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 0.234 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Recombinant Human IL-2R beta/CD122 Protein, mFc Tag (Cat. No. 10696-H05H) captured on CM5 chip via anti-mouse antibodies surface can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 0.460 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 6.Loaded Biotinylated Recombinant Human IL-2Rβ & IL-2Rγ Protein, Flag & AVI & His Tag (Cat. No. CT108-H2549H1-B) on SA Biosensor, can bind Recombinant Human IL-2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 2.190 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ala21-Thr153
Molecular Weight
The recombinant human IL-2 consists of 134 amino acids and predicts a molecular mass of 15.55 kDa.
Species
Human

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