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IL4 Protein (Native, Human)

CAT: 1543-P520311Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P520311Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IL4
Synonyms
BCGF-1, BCGF1, BSF-1, BSF1, IL-4, MGC79402
Host
E. coli
Tag
Native
Endotoxin
< 5 EU per mg of the protein.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Measured in a cell proliferation assay using TF‐1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of recombinant human IL-4 is >10,000 IU/μg which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656) . 2.Dendritic Cells (DCs) generation and maturation. CD14+ monocytes were differentiated into immature DCs by GM-CSF (Cat#GMP-10015-HNAH) and IL4 (Cat#GMP-11846-HNAE) for 6 days. DCs were matured by TNF (Cat#GMP-10602-HNAE) for 2 days. (Routinely tested) 3.Dendritic Cells (DCs) generation. CD14+ monocytes were differentiated into immature DCs by GM-CSF (Cat#GMP-10015-HNAH) and IL4 (Cat#GMP-11846-HNAE) for 6 days. (Routinely tested)
Function
His25-Ser153
Molecular Weight
The recombinant human IL4 consists of 130 amino acids and migrates with an apparent molecular mass of 15.1 kDa as predicted.
Species
Human

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