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CD28 Protein (C-His, Human)

CAT: 1543-P520240Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P520240Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
CD28
Synonyms
Tp44
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity
1.Binding activity of PE-conjugated CD28 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human CD28 Protein (Site-Specific PE Conjugation / His Tag, Cat. No. 11524-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 2.1E5 of mouse Anti-CD28 antibody-conjugated beads (6-8 μm) were stained with a series of concentrations of PE-conjugated human CD28 Protein (Site-Specific PE Conjugation / His Tag, Cat. No. 11524-H86H) . Binding activity was quantified based on PE fluorescence. The recommended usage is 2 μL per 100 μL staining volume (end-user titration is recommended for optimal performance) .
Function
Asn19-Pro152
Molecular Weight
The protein has a predicted molecular mass of 18.5 kDa.
Species
Human