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CD4 Protein (C-His, Human)

CAT: 1543-P520029Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P520029Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
CD4
Synonyms
CD4mut, IMD79, Leu-3, OKT4D, T4
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 90 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to CD4-coated plates (0.8 μg/mL, 100 μL/well), approximately >40% will adhere specifically (Routinely tested) . 2.Immobilized Anti-CD4 (Research grade Ibalizumab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD4 / LEU3 Protein (His Tag) (Cat: 10400-H08H), the EC50 is 0.6-2.5 ng/mL (QC tested) . 3.Immobilized Anti-CD4 (Research Grade Ibalizu*mab Biosimilar) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD4 / LEU3 Protein (His Tag) (Cat: 10400-H08H), the EC50 is 3.2 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human CD4 / LEU3 Protein (His Tag) (Cat: 10400-H08H) at 1 μg/mL (100 μL/well) can bind Anti-CD4 (Research Grade Ibalizu*mab Biosimilar), the EC50 is 11.0 ng/mL (Routinely tested) .
Function
Met1-Trp390
Molecular Weight
The recombinant human CD4 consists of 376 amino acids and has a predicted molecular mass of 42.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD4 is approximately 46 kDa due to glycosylation.
Species
Human

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