Apolipoprotein E/APOE3, Human (HEK293, His)

Apolipoprotein E/APOE3, Human (HEK293, His)

• UNSPSC Description: Apolipoprotein E/ApoE3 is an apolipoprotein isoform that mediates lipid transport and plays a crucial role in lipid homeostasis in both plasma and the central nervous system by binding to LDL receptors (LDLR), LDL receptor-related proteins (LRP1, LRP2, LRP8), Very Low-Density Lipoprotein Receptor (VLDLR), and Heparin. The Apolipoprotein E/ApoE3 Protein, Human (HEK293, His) is a recombinant protein with a His tag at the N-terminus, consisting of 299 amino acids (K19-H317), and is expressed in HEK293 cells[1].
• Type: Recombinant Proteins
• Assay Protocol: https://www.medchemexpress.com/cytokines/apolipoprotein-e-apoe-protein-human-hek293-his.html
• Purity: 98.0
• Smiles: KVEQAVETEP EPELRQQTEW QSGQRWELAL GRFWDYLRWV QTLSEQVQEE LLSSQVTQEL RALMDETMKE LKAYKSELEE QLTPVAEETR ARLSKELQAA QARLGADMED VCGRLVQYRG EVQAMLGQST EELRVRLASH LRKLRKRLLR DADDLQKRLA VYQAGAREGA ERGLSAIRER LGPLVEQGRV RAATVGSLAG QPLQERAQAW GERLRARMEE MGSRTRDRLD EVKEQVAEVR AKLEEQAQQI RLQAEAFQAR LKSWFEPLVE DMQRQWAGLV EKVQAAVGTS AAPVPSDNH
• Molecular Weight: 36-39 kDa
• References & Citations: [1]Gong JS, et al. Apolipoprotein E (ApoE) isoform-dependent lipid release from astrocytes prepared from humanApoE3 and ApoE4 knock-in mice. J Biol Chem. 2002 Aug 16;277(33):29919-26.|[2]Gong JS, et al. Novel action of apolipoprotein E (ApoE): ApoE isoform specifically inhibits lipid-particle-mediated cholesterol release from neurons. Mol Neurodegener. 2007 May 15;2:9.|[3]Hatters DM, et al. Apolipoprotein E structure: insights into function. Trends Biochem Sci. 2006 Aug;31(8):445-54.|[4]Wetterau JR, et al. Human apolipoprotein E3 in aqueous solution. I. Evidence for two structural domains. J Biol Chem. |[5]Dorey E, et al. Apolipoprotein E Isoforms Differentially Regulate Alzheimer's Disease and Amyloid-β-Induced Inflammatory Response in vivo and in vitro. J Alzheimers Dis. 2017;57(4):1265-1279.|[6]Sullivan PM, et al. Targeted replacement of the mouse apolipoprotein E gene with the common human APOE3 allele enhances diet-induced hypercholesterolemia and atherosclerosis. J Biol Chem.|[7]Huang Y W A, et al. ApoE2, ApoE3, and ApoE4 differentially stimulate APP transcription and Aβ secretion[J]. Cell, 2017, 168(3): 427-441. e21.|[8]Gong J S, et al. Apolipoprotein E (ApoE) isoform-dependent lipid release from astrocytes prepared from human ApoE3 and ApoE4 knock-in mice[J]. Journal of Biological Chemistry, 2002, 277(33): 29919-29926.|[9]Sehayek E, et al. Mechanisms of inhibition by apolipoprotein C of apolipoprotein E-dependent cellular metabolism of human triglyceride-rich lipoproteins through the low density lipoprotein receptor pathway.|[10]Kowal RC, et al. Low density lipoprotein receptor-related protein mediates uptake of cholesteryl esters derived from apoprotein E-enriched lipoproteins. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5810-4.|[11]Ji ZS, et al. Heparan sulfate proteoglycans participate in hepatic lipaseand apolipoprotein E-mediated binding and uptake of plasma lipoproteins, including high density lipoproteins. J Biol Chem.|[12]Fagan AM, et al. Apolipoprotein E-containing high density lipoprotein promotes neurite outgrowth and is a ligand for the low density lipoprotein receptor-related protein. J Biol Chem. 1996 Nov 22;271(47):30121-5.|[13]Deng M, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018 Oct;562(7728):605-609. |[14]Huang YA, et al. ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion. Cell. 2017 Jan 26;168(3):427-441.e21.
• Shipping Conditions: Blue Ice
• Storage Conditions: Stored at -20°C for 2 years