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INT

CAT: 0804-HY-15920-02Size: 10 mM - 1 mLDry Ice: NoHazardous: No
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CAT#:0804-HY-15920-02Size:10 mM - 1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
INT (Iodonitrotetrazolium chloride) is a biochemical reagent mainly used in the iodonitrotetrazolium chloride (INT) colorimetric method to assess the metabolic activity of cells and the activity of enzymes[1].
CAS Number
146-68-9
Product Name Alternative
Iodonitrotetrazolium chloride; p-Iodonitrotetrazolium Violet
UNSPSC
12352200
Hazard Statement
H228, H335
Target
Biochemical Assay Reagents
Type
Biochemical Assay Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/int.html
Purity
99.57
Solubility
DMSO : 83.33 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL (ultrasonic; warming; heat to 60°C)
Smiles
IC1=CC=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=C([N+]([O-])=O)C=C4)C=C1.[Cl-]
Molecular Formula
C19H13ClIN5O2
Molecular Weight
505.70
Precautions
H228, H335
References & Citations
[1]Munujos P, et al. Assay of succinate dehydrogenase activity by a colorimetric-continuous method using iodonitrotetrazolium chloride as electron acceptor[J]. Analytical biochemistry, 1993, 212 (2) : 506-509.|[2]Von Mersi W, et al., An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride[J]. Biology and fertility of soils, 1991, 11: 216-220.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, sealed storage, away from moisture)
Scientific Category
Biochemical Assay Reagents
Clinical Information
No Development Reported

Chemical Information

Iodonitrotetrazolium violet

Iodonitrotetrazolium chloride is an organic chloride salt having iodonitrotetrazolium as the counterion. It has a role as a histological dye. It contains an iodonitrotetrazolium.

CAS Number146-68-9
PubChem CID64957
IUPAC Name2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazol-3-ium chloride
Molecular FormulaC19H13ClIN5O2
Molecular Weight505.7
Topological Polar Surface Area80.4
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count5
Rotatable Bond Count3
Heavy Atom Count28
Formal Charge0
Complexity497
SMILES
C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=C(C=C3)[N+](=O)[O-])C4=CC=C(C=C4)I.[Cl-]
InChI
InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChIKey
JORABGDXCIBAFL-UHFFFAOYSA-M
Chemical Structure
2D Structure
2D structure of Iodonitrotetrazolium violet
CAS: 146-68-9
CID: 64957
Formula: C19H13ClIN5O2
MW: 505.7
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight505.7
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count5
Rotatable Bond Count3
Exact Mass504.98025
Monoisotopic Mass504.98025
Topological Polar Surface Area80.4 Ų
Heavy Atom Count28
Formal Charge0
Complexity497
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count2
Compound Is CanonicalizedYes

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