Human DUSP10 Recombinant Protein

• Description :

  Human DUSP10 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 149-482aa. Application (s) : SDS-PAGE, Enzyme Activity.

• Synonyms :

  Dual specificity protein phosphatase 10, MKP-5, MKP5, Mitogen-activated protein kinase phosphatase 5, MAP kinase phosphatase 5

• Gene Name :

  DUSP10

• UniProt :

  Q9Y6W6

• Tag :

  His-Tag

• Source :

  E. coli

• Applications :

  SDS-PAGE, Enzyme Activity

• Field of Research :

  Enzymes and Proteases

• Purification :

  > 90% by SDS-PAGE

• Concentration :

  0.25mg/mL (determined by Bradford assay)

• Bioactivity :

  Specific activity is > 600 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.

• Form :

  Liquid

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

• Observed Molecular Weight :

  40.4 kDa (359aa) confirmed by MALDI-TOF

• Contents :

  20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 200mM NaCl