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Mouse Pgk1 Recombinant Protein

CAT: 0519-PROTP09411-100ugSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTP09411-100ugSize:100 µg
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Description
Mouse Pgk1 Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-417aa. Application (s) : Enzyme Activity, SDS-PAGE.
Synonyms
Phosphoglycerate kinase 1, Pgk-1
Gene Name
Pgk1
UniProt
P09411
Tag
His-Tag
Source
E. coli
Applications
Enzyme Activity, SDS-PAGE
Field of Research
Cardiovascular
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by Bradford assay)
Bioactivity
Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
47.1 kDa (441aa) confirmed by MALDI-TOF
Contents
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

UniProtKB · P09411

Phosphoglycerate kinase 1

PGK1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P09411
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pgk1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
2.7.11.1, 2.7.2.3
Processing
—
Secondary accessions
Q3TPE6, Q3UKV8, Q5XJE7
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

AcetylationHydroxylationPhosphoprotein

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

CytoplasmMitochondrion

Biological process

Glycolysis

Molecular function

KinaseTransferase

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