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Mouse TNF-alpha Recombinant Protein

CAT: 0519-PROTP06804-100ugSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTP06804-100ugSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse TNF-alpha Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 80-235aa. Application (s) : SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .
Synonyms
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Gene Name
Tnf
UniProt
P06804
Tag
His-Tag
Source
Baculovirus
Applications
SDS-PAGE, Bioactivity
Field of Research
Hormones and Cytokines
Purification
> 90% by SDS-PAGE
Concentration
1mg/mL (determined by absorbance at 280nm)
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range is less or equal to 0.02 ng/mL.
Form
Liquid
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Observed Molecular Weight
18kDa (162aa)
Contents
Phosphate-Buffered Saline (pH 7.4)

UniProtKB · P06804

Tumor necrosis factor

TNFA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06804
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tnf
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O35853, Q62326, Q91VF3
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Cellular component

Cell membraneMembraneSecreted

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinLipoproteinMyristatePhosphoprotein

Domain

Signal-anchorTransmembraneTransmembrane helix

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