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Anti-RNF20 Antibody

CAT: 0519-M03457-2-50ulSize: 50 µLDry Ice: NoHazardous: No
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CAT#:0519-M03457-2-50ulSize:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1) . H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively) . It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Description
Boster Bio Anti-RNF20 Antibody (Catalog # M03457-2) . Tested in WB application (s) . This antibody reacts with Human.
Synonyms
E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, 632-, RING finger protein 20, RNF20, BRE1A
Gene Name
RNF20
UniProt
Q5VTR2
Host
Mouse
Reactivity
Human
Immunogen
This RNF20 antibody is generated from a mouse immunized with a recombinant protein of human RNF20.
Clonality
Monoclonal
Clone
Clone: 1594CT552.128.36
Tissue Specificity
Expressed in the normal brain and also in malignant gliomas (at protein level) .
Applications
WB
Field of Research
Cell Biology
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1) . H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively) . It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
113662 Da
Applications Notes
WB: 1:2000
Subcellular Location
Nucleus
Isotype
IgG1, k
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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