Products for Research Use Only
RegisterLogin

Anti-ECT2 Antibody

CAT: 0519-M01862-50ulSize: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0519-M01862-50ulSize:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB) - induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Description
Boster Bio Anti-ECT2 Antibody (Catalog # M01862) . Tested in WB application (s) . This antibody reacts with Human.
Synonyms
Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2
Gene Name
ECT2
UniProt
Q9H8V3
Host
Mouse
Reactivity
Human
Immunogen
This ECT2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 200-460 amino acids from human ECT2.
Clonality
Monoclonal
Clone
Clone: 1691CT516.3.3
Tissue Specificity
Expressed in lung epithelial cells (at protein level) . Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma
Applications
WB
Field of Research
Cell Biology
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Function
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB) -induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A- PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
103505 Da
Applications Notes
WB: 1:1000
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Sequestered within the nucleus during interphase (PubMed:10579713) . Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis (PubMed:10579713) . Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis (PubMed:10579713) . Colocalized with RhoA at the midbody (PubMed:10579713) . Its subcellular localization to tight junction is increased by calcium (PubMed:15254234) .
Isotype
IgG1, k
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Related Products

CatalogName
P01862Anti-Phospho-ECT2 T790 Antibody
M01862-1Anti-ECT2 Mouse Monoclonal Antibody [Clone ID: OTI2D9]
LS001894ECT2 (NM_018098) Human Over-expression Lysate
LS001894-20ugECT2 (NM_018098) Human Over-expression Lysate
LS001894-100ugECT2 (NM_018098) Human Over-expression Lysate
A13893Anti-Ecto-ADP-ribosyltransferase 5 ART5 Antibody
A09597-2Anti-ANT4/SLC25A31 Antibody
A06393-1Anti-ANT1/2 Antibody
A09597-2-carrier-freeAnti-ANT4/SLC25A31 Antibody
A09597-1Anti-ANT4 SLC25A31 Antibody

Alternative Products

CatalogName
STJ70379Anti-ECT2 antibody
abx457127-01Protein ECT2 (ECT2) Antibody
SL23903R-01Rabbit Anti-ECT2 antibody
dAP-0214Goat anti-ECT2 Antibody
HV384014-01Anti-ECT2 Polyclonal Antibody
GWB-208397Anti-ECT2 pT790 Antibody
TMAB-05428-01Anti-ECT2 Antibody (8O688)
SLM-51635M-01Mouse Anti-ECT2 antibody
GWB-MS591FECT2 Antibody

Popular Products