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Anti-IF4E Antibody

CAT: 0519-M00135-2-80ulSize: 80 µLDry Ice: NoHazardous: No
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CAT#:0519-M00135-2-80ulSize:80 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Description
Boster Bio Anti-IF4E Antibody (Catalog # M00135-2) . Tested in WB, IF application (s) . This antibody reacts with Human, Mouse, Rat.
Synonyms
Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Gene Name
EIF4E
UniProt
P06730
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.
Clonality
Monoclonal
Clone
Clone: 1504CT774.14.55
Applications
WB, IF
Field of Research
Viral process, Host-Virus Interaction
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Function
116062002433528529987188226840102257881316271312225788132998718824335285243352852347107822684010
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
25097 Da
Subcellular Location
Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus. Nucleus speckle. Nucleus, nuclear body Note=Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732) . Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257) Sequestered in the nucleus by EIF4EBP1 and EIF4EBP2 (By similarity) {ECO:0000250|UniProtKB:P63073, ECO:0000269|PubMed:10856257, ECO:0000269|PubMed:16157702, ECO:0000269|PubMed:24335285, ECO:0000269|PubMed:25923732}
Isotype
IgG1, Κ
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

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