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Anti-AFAP1 Antibody

CAT: 0519-A05258Size: 0.1 mgDry Ice: NoHazardous: No
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CAT#:0519-A05258Size:0.1 mg
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Background
The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.
Description
Boster Bio Anti-AFAP1 Antibody (Catalog # A05258) . Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Synonyms
AFAP, AFAP110, AFAP-110, AFAP, Actin filament-associated protein 1, 110 kDa actin filament-associated protein
Gene Name
AFAP1
UniProt
Q8N556
Host
Rabbit
Reactivity
Human, Mouse
Cross Reactivity
Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.
Immunogen
AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1. The immunogen is located within amino acids 30 - 80 of AFAP1.
Clonality
Polyclonal
Tissue Specificity
Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF- 7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
Applications
ELISA, WB
Field of Research
Cancer, Cell Cycle
Purification
AFAP1 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL
Form
Liquid
Function
Can cross-link actin filaments into both network and bundle structures (By similarity) . May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Storage Conditions
AFAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Observed Molecular Weight
80725 MW
Gene Name Synonym
Actin filament associated protein 1
Subcellular Location
Cytoplasm, cytoskeleton
Prediction Reactivity
Rat
Protein Name
Actin filament associated protein 1
Isotype
IgG
Contents
AFAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

UniProtKB · Q8N556

Actin filament-associated protein 1

AFAP1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8N556
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AFAP1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K442, B4DMU2, E9PDT7, Q59EY5, Q9HBY1
Protein keywords

PTM

AcetylationPhosphoprotein

Molecular function

Actin-binding

Coding sequence diversity

Alternative splicing

Domain

Coiled coilRepeat

Cellular component

CytoplasmCytoskeleton

Technical term

Proteomics identificationReference proteome

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