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Human GIT1 ELISA Kit PicoKine®

CAT: 0519-EK2311Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK2311Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts ly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology.
Description
Human GIT1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GIT1 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 44 33 53 41 3.25 7.9% Sample 2 218 200 197 238 213 14.35 6.7% Sample 3 601 969 744 549 716 17.97 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 44 33 53 41 3.25 7.9% Sample 1 35 44 33 53 41 3.25 7.9% Sample 2 218 200 197 238 213 14.35 6.7% Sample 2 218 200 197 238 213 14.35 6.7% Sample 3 601 969 744 549 716 17.97 2.5% Sample 3 601 969 744 549 716 17.97 2.5%
Synonyms
DNA repair protein XRCC2; X-ray repair cross-complementing protein 2; Xrcc2
Gene Name
GIT1
Gene ID
Q9Y2X7
UniProt
Q9Y2X7
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Capture Antibody
Monoclonal antibody from goat
Detection Antibody
Monoclonal antibody from goat
Tissue Specificity
Expressed at low level in somatic tissues and at high level in testis.
Applications
ELISA
Field of Research
Signal Transduction
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA)
Detection Range
31.2-2,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2%
Sensitivity
<5 pg/mL
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
84341 MW
Specificity
Natural and recombinant human GIT1
Anticoagulant
Heparin or EDTA
Gene Name Synonym
X-ray repair complementing defective repair in Chinese hamster cells 2
Subcellular Location
Nucleus.
Protein Name
DNA repair protein XRCC2
Sample Data
Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05

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