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Mouse Cripto/TDGF1 ELISA Kit PicoKine®

CAT: 0519-EK1405Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK1405Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
Description
Mouse Cripto/TDGF1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tdgf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 110 114 115 112 2.27 2% Sample 2 661 633 642 685 655 19.92 3% Sample 3 1213 1047 1166 1231 1164 71.73 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 110 114 115 112 2.27 2% Sample 1 110 110 114 115 112 2.27 2% Sample 2 661 633 642 685 655 19.92 3% Sample 2 661 633 642 685 655 19.92 3% Sample 3 1213 1047 1166 1231 1164 71.73 6.1% Sample 3 1213 1047 1166 1231 1164 71.73 6.1%
Synonyms
Teratocarcinoma-derived growth factor; Cripto growth factor; Epidermal growth factor-like Cripto protein; Tdgf1; Cripto
Gene Name
Tdgf1
Gene ID
21667
UniProt
P51865
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: R26-Q150
Capture Antibody
Monoclonal antibody from rat
Detection Antibody
Polyclonal antibody from goat
Tissue Specificity
Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.
Applications
ELISA
Field of Research
Embryonic Stem Cells, Lineage Markers, Lineage Specification, Secreted, Stem Cells
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
Detection Range
62.5 pg/mL - 4,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5%
Sensitivity
<10 pg/mL
Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
18754 MW
Specificity
Natural and recombinant mouse Cripto
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Teratocarcinoma-derived growth factor
Subcellular Location
Cell membrane ; Lipid-anchor, GPI-anchor .
Protein Name
Teratocarcinoma-derived growth factor
Sample Data
Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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