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Mouse CD30L ELISA Kit PicoKine®

CAT: 0519-EK0572Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK0572Size:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The human CD30L (CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that thisreverse signaling modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.
Description
Mouse CD30L ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 30 34 31 32 2.06 6.4% Sample 2 254 260 260 224 249 14.92 5.9% Sample 3 445 507 470 490 478 23.11 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 30 34 31 32 2.06 6.4% Sample 1 35 30 34 31 32 2.06 6.4% Sample 2 254 260 260 224 249 14.92 5.9% Sample 2 254 260 260 224 249 14.92 5.9% Sample 3 445 507 470 490 478 23.11 4.8% Sample 3 445 507 470 490 478 23.11 4.8%
Synonyms
Tumor necrosis factor ligand superfamily member 8; CD30 ligand; CD30-L; CD153; Tnfsf8; Cd30l, Cd30lg
Gene Name
TNFSF8
Gene ID
21949
UniProt
P32972
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q68-D239
Capture Antibody
Monoclonal antibody from rat
Detection Antibody
Polyclonal antibody from goat
Applications
ELISA
Field of Research
Adaptive Immunity, Immunology, T Cells
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
Detection Range
15.6 pg/mL - 1,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 254 445 38 267 477 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 254 445 38 267 477 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 254 445 38 267 477 Mean (pg/ml) 35 254 445 38 267 477 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1% CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1%
Sensitivity
<6 pg/mL
Function
Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
26519 MW
Specificity
Natural and recombinant mouse CD30L
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Tumor necrosis factor ligand superfamily member 8
Subcellular Location
Membrane; Single-pass type II membrane protein.
Protein Name
Tumor necrosis factor ligand superfamily member 8
Sample Data
Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

UniProtKB · P32972

Tumor necrosis factor ligand superfamily member 8

TNFL8_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P32972
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tnfsf8
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Cellular component

Membrane

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix

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