Products for Research Use Only
RegisterLogin

Mouse CD44 PicoKine® Quick ELISA Kit

CAT: 0519-FEK1419Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0519-FEK1419Size:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Description
Mouse CD44 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse CD44 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 778 783 658 694 728 31.31 4.3% Sample 2 1941 1958 1941 2136 1994 69.79 3.5% Sample 3 8310 7950 8440 8644 8336 233.42 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 778 783 658 694 728 31.31 4.3% Sample 1 778 783 658 694 728 31.31 4.3% Sample 2 1941 1958 1941 2136 1994 69.79 3.5% Sample 2 1941 1958 1941 2136 1994 69.79 3.5% Sample 3 8310 7950 8440 8644 8336 233.42 2.8% Sample 3 8310 7950 8440 8644 8336 233.42 2.8%
Gene Name
Cd44
Gene ID
12505
UniProt
P15379
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: CHO; Immunogen sequence: Q25-T224
Capture Antibody
Polyclonal antibody from goat
Detection Antibody
Polyclonal antibody from goat
Applications
ELISA
Standard
20 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
312 pg/mL - 20,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 778 1941 8310 763 1853 7959 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 778 1941 8310 763 1853 7959 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 778 1941 8310 763 1853 7959 Mean (pg/ml) 778 1941 8310 763 1853 7959 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 Standard deviation 43.57 137.81 623.25 52.65 120.45 541.21 CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8% CV (%) 5.6% 7.1% 7.5% 6.9% 6.5% 6.8%
Sensitivity
<10 pg/mL
Components
Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
Storage Conditions
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Specificity
Natural and recombinant Mouse Cd44
Anticoagulant
Heparin or EDTA
Sample Data
Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161 O.D. 0.029 0.071 0.162 0.277 0.471 0.868 1.470 2.161
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

UniProtKB · P15379

CD44 antigen

CD44_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P15379
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cd44
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q05732, Q61395, Q62060, Q62061, Q62062, Q62063, Q62408, Q62409, Q64296, Q99J14, Q9QYX8
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Cellular component

Cell membraneCell projectionMembraneSecreted

PTM

Disulfide bondGlycoproteinPhosphoproteinProteoglycanSulfation

Molecular function

Receptor

Domain

SignalTransmembraneTransmembrane helix

Related Products

CatalogName
FEK1418Human CD44 PicoKine® Quick ELISA Kit
FEK1417Rat CD44 PicoKine® Quick ELISA Kit
FEK1418-carrier-freeHuman CD44 PicoKine® Quick ELISA Kit, Carrier-free
FEK1417-carrier-freeRat CD44 PicoKine® Quick ELISA Kit, Carrier-free
EK1419Mouse CD44 ELISA Kit PicoKine®
FEK0703Mouse CD40/TNFRSF5 PicoKine® Quick ELISA Kit
FEK0309Mouse BDNF PicoKine® Quick ELISA Kit
FEK0808Mouse CSF1R PicoKine® Quick ELISA Kit
FEK1384Mouse ADAMTS1 PicoKine® Quick ELISA Kit
FEK1287Mouse CD6 PicoKine® Quick ELISA Kit

Alternative Products

CatalogName
MBS1759922-01Human CD44 PicoKine Quick ELISA Kit
FEK1418Human CD44 PicoKine® Quick ELISA Kit
MBS1756435-01Mouse CD44 PicoKine Quick ELISA Kit
FEK1419Mouse CD44 PicoKine® Quick ELISA Kit
FEK1417Rat CD44 PicoKine® Quick ELISA Kit
GTR18860973-01Rat Cd44 (CD44 antigen) Quick ELISA Kit
EK1419Mouse CD44 ELISA Kit PicoKine®
MBS177561-01Mouse CD44 PicoKine ELISA Kit
EK1417Rat CD44 ELISA Kit PicoKine®
MBS177555-01Rat CD44 PicoKine ELISA Kit

Popular Products