Anti-Cd4 Antibody

CAT: 0519-A00344-3Size: 100 µg/VialDry Ice: NoHazardous: No

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CAT#:0519-A00344-3Size:100 µg/Vial

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of in neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Description

Boster Bio Anti-Cd4 Antibody Picoband® catalog # A00344-3. Tested in ELISA, IHC applications. This antibody reacts with Rat.

Synonyms

Beta-nerve growth factor; Beta-NGF; NGF; NGFB

Gene Name

Cd4

Gene ID

24932

UniProt

P05540

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived rat Cd4 recombinant protein (Position: K28-A246) .

Clonality

Polyclonal

Tissue Specificity

Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen) . Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

Applications

IHC, ELISA

Field of Research

Growth and Development, Mesenchymal Stem Cells, Neural Stem Cells, Neurology Process, Neuroscience, Stem Cells, Surface Molecules

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) . The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) . Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form.

References & Citations

1. Ansari-Lari, M. A., Muzny, D. M., Lu, J., Lu, F., Lilley, C. E., Spanos, S., Malley, T., Gibbs, R. A. A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13. Genome Res. 6: 314-326, 1996. 2. Ben-Sasson, S. Z., Hu-Li, J., Quiel, J., Cauchetaux, S., Ratner, M., Shapira, I., Dinarello, C. A., Paul, W. E. IL-1 acts ly on CD4 T cells to enhance their antigen-driven expansion and differentiation. Proc. Nat. Acad. Sci. 106: 7119-7124, 2009. 3. Browning, J., Horner, J. W., Pettoello-Mantovani, M., Raker, C., Yurasov, S., DePinho, R. A., Goldstein, H. Mice transgenic for human CD4 and CCR5 are susceptible to HIV infection. Proc. Nat. Acad. Sci. 94: 14637-14641, 1997.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

26959 MW

Observed Molecular Weight

52 kDa

Gene Name Synonym

Nerve growth factor

Subcellular Location

Secreted. Endosome lumen.

Protein Name

Beta-nerve growth factor

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

UniProtKB · P05540

T-cell surface glycoprotein CD4

CD4_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: P05540
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Cd4
Protein existence: 1: Evidence at protein level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

Adaptive immunityImmunity

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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