Anti-CD74 Antibody

CAT: 0519-A01340-2Size: 100 µg/VialDry Ice: NoHazardous: No

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CAT#:0519-A01340-2Size:100 µg/Vial

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

HLA class II histocompatibility antigen gamma chain also known as HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74), is a protein that in humans is encoded by the CD74 gene. The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Description

Boster Bio Anti-CD74 Antibody Picoband® catalog # A01340-2. Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human, Mouse, Rat.

Synonyms

Metabotropic glutamate receptor 5; mGluR5; GRM5; GPRC1E, MGLUR5

Gene Name

CD74

Gene ID

972

UniProt

P04233

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD74, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level) . Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.

Applications

Flow Cytometry, IF, IHC, ICC

Field of Research

Subcellular Markers, Tags & Cell Markers, Vesicle Transport

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.

References & Citations

1. Claesson, L., Larhammar, D., Rask, L., Peterson, P. A. cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure. Proc. Nat. Acad. Sci. 80: 7395-7399, 1983. 2. Claesson-Welsh, L., Barker, P. E., Larhammar, D., Rask, L., Ruddle, F. H., Peterson, P. A. The gene encoding the human class II antigen-associated gamma chain is located on chromosome 5. Immunogenetics 20: 89-93, 1984. 3. Driessen, C., Bryant, R. A., Lennon-Dumenil, A. M., Villadangos, J. A., Bryant, P. W., Shi, G. P., Chapman, H. A., Ploegh, H. L. Cathepsin S controls the trafficking and maturation of MHC class II molecules in dendritic cells. J. Cell Biol. 147: 775-790, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

178 kDa

Applications Notes

Gene Name Synonym

Glutamate metabotropic receptor 5

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

UniProtKB · P04233

HLA class II histocompatibility antigen gamma chain

HG2A_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P04233
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CD74
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: A8K7R1, B4DNE8, D3DQG3, D3DQG4, Q14597, Q29832, Q5U0J8, Q8SNA0, Q8WLP6

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative initiationAlternative splicing

Cellular component

Cell membraneEndoplasmic reticulumEndosomeGolgi apparatusLysosomeMembraneSecreted

Molecular function

Chaperone

PTM

Disulfide bondGlycoproteinPhosphoproteinProteoglycan

Domain

Signal-anchorTransmembraneTransmembrane helix

Related Products

Catalog	Name
M01340-4	Anti-CD74 Reference Antibody (milatuzumab)
M01340-2	Anti-CD74 Rabbit Monoclonal Antibody
M01340-2-carrier-free	Anti-CD74 Rabbit Monoclonal Antibody, Carrier-free
M01340-1	Anti-CD74 (B-Cell Marker) Monoclonal Antibody
PROTP04233-50ug	Human CD74 Recombinant Protein
PROTP04233-250ug	Human CD74 Recombinant Protein
PROTP04233-10ug	Human CD74 Recombinant Protein
FC01340-PE-Cy7-01	Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated
EK2152	Human CD74 ELISA Kit PicoKine®
PROTP04233-1	Human, CD74 Human Recombinant Protein, Sf9

Alternative Products

Catalog	Name
MBS9510642-01	Anti-CD74 Antibody [ABT-CD74]
GTR18088646	Anti-CD74 Antibody