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PCNA Polyclonal Antibody

CAT: 0749-ABP0112Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP0112Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Proliferating cell nuclear antigen encoded by PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. Proliferating cell nuclear antigen acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, proliferating cell nuclear antigen is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
Description
Rabbit Anti-PCNA Polyclonal Antibody
Product Name Alternative
PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin
Gene ID
5111
Accession Number
P12004
Reactivity
Human, Mouse, Rat, Monkey, Rabbit
Immunogen
Synthesized peptide derived from the Internal region of human PCNA at AA range: 30-110
Clonality
Polyclonal
Tag
Proliferating cell nuclear antigen
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
30-33kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-500; IF/ICC1:100-500; ELISA 1:5000-20000
Other Product Names
PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P12004
Isotype
Rabbit IgG

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