Recombinant Rat TNFA

CAT: 0710-P4812-01Size: 50 µgDry Ice: NoHazardous: No

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CAT#:0710-P4812-01Size:50 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, Tnf, TNF superfamily member 2, TNF superfamily, member 2, TNF

UniProt

P16599

Expression Region

80-235

Host

E.Coli

Tag

N-terminal His Tag

Applications

Western Blot, ELISA

Endotoxin

< 1 EU/µg as determined by LAL test.

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 10-30 pg/mL.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

17.1 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

Organism Species

Rat

UniProtKB · P16599

Tumor necrosis factor

TNFA_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: P16599
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Tnf
Protein existence: 2: Evidence at transcript level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: Q6EE11, Q9JI26, Q9JI27

Protein keywords

Cellular component

Cell membraneMembraneSecreted

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinLipoproteinMyristatePhosphoprotein

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix

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