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Exonuclease III

CAT: 1465-EG20207SSize: 5000 UDry Ice: NoHazardous: No
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CAT#:1465-EG20207SSize:5000 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Exonuclease III is a nuclease that stepwise removes mononucleotides from the 3'-OH termini of double-stranded DNA (dsDNA), and inactive on single-stranded DNA (ssDNA) . The preferred substrates of Exonuclease III are blunt or recessed 3'-termini, although the enzyme also acts at nick sites in double-stranded DNA to generate single-stranded gaps. 3'-overhanging termini are resistant to cleavage; the degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This characteristic can be used to produce strand-specific ssDNA with designed linearized dsDNA having one non-cleavage end (3'-overhang) and the other end designed as a cleavable end (blunt or 5'-overhang), allowing Exonuclease III to digest only one strand. Exonuclease III also exhibits RNase H, 3'-phosphatase, and apurinic/apyrimidinic-endonuclease activities.
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG20207.pdf
Concentration
100 U/μL
Components
• Exonuclease III (100 U/μl) : 50 μl • 10xExo III Buffer: 500 μl
Shipping Conditions
2~8°C
Storage Conditions
-20°C
Shelf Life
2 Years

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