DsDNase

CAT: 1465-EG20206SSize: 50 ReactionsDry Ice: NoHazardous: No

1 / 1

CAT#:1465-EG20206SSize:50 Reactions

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Description

DsDNase is a deoxyribonuclease that hydrolyzes phosphodiester bonds in dsDNA, generating oligonucleotides with 5'-phosphate and 3'-hydroxyl ends. dsDNase specifically hydrolyzes dsDNA or the DNA strand in DNA-RNA hybrid chains, while having no effect on RNA and single-stranded DNA. dsDNase is heat-labile and can be rapidly inactivated at 55 °C with DTT. It is primarily used to eliminate genomic DNA contamination in RNA samples before reverse transcription. Compared to the traditional method of using DNase I to eliminate genomic DNA contamination, dsDNase does not require additional EDTA, reduces RNA damage, saves experimental time, and ensures accurate quantification of RNA. The activity of dsDNase can be inhibited by EDTA, SDS, DTT, β-mercaptoethanol and high salt concentrations.

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG20206-1.pdf

Concentration

1 Reaction/μL

Components

• dsDNase (1 rxn/μl) : 50 μl • 10xdsDNase Buffer: 200 μl

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

Alternative Products

Catalog	Name
TRP-00503-01	DsDNase
B2016565	DsDNase Enzyme
D7078S	Thermolabile dsDNase
G3408-50UL	DsDNase (Thermolabile)
BAL912	DsDNase, 1 pack = 50 rxns
8072041	All-in-One First-Strand Synthesis (+dsDNase)
SR514	UnionScript First-strand cDNA Synthesis kit (with dsDNase)
M127-01	HeatLabile Double-Strand Specific DNase (HL-dsDNase)
SR511	UnionScript First-strand cDNA Synthesis Mix for qPCR (with dsDNase)
E2030	Atlantis dsDNase 12.5 U (0.1 U/ul)

DsDNase

Alternative Products

Popular Products