Alkaline Phosphatase (Fast)

CAT: 1465-EG15208SSize: 1000 UDry Ice: NoHazardous: No

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CAT#:1465-EG15208SSize:1000 U

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Alkaline Phosphatase (Fast) is an enzyme that catalyzes the hydrolysis of the 5' and 3' phosphate groups of DNA, RNA, and nucleotides. It can also remove protein phosphate groups but does not hydrolyze phosphodiester and phosphotriester bonds. This enzyme can dephosphorylate the ends of all types of DNA in 10 minutes at 37°C . It has 100% activity in CutOneTM buffer and is inactivated by incubation at 80 °C for 20 minutes. When used together with LightiNingTM restriction endonucleases, it simplifies the "digestion-dephosphorylation" reaction in one tube.

Assay Protocol

http://en.best-enzymes.com/storage/upload/files/directions/05xiushimei/EG15208.pdf

Concentration

1 U/μL

Components

• Alkaline Phosphatase (Fast) (1 U/μl) : 1000 μl • 10x AP Buffer: 2x1 ml

Shipping Conditions

2~8°C

Storage Conditions

-20°C

Shelf Life

2 Years

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Alkaline Phosphatase (Fast)

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