BbvCI

CAT: 1465-EG25516SSize: 50 UDry Ice: NoHazardous: No
CAT#:1465-EG25516SSize:50 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BbvCI is derived from the BbvCI gene of Bacillus brevis (L. Ge) and is obtained through recombinant expression in Escherichia coli. BbvCI is a Type IIA restriction enzyme existing as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site. It recognizes and cleaves the non-palindromic sequence CCTCAGC (-5/-2), generating 5' cohesive ends with 3-base overhangs. BbvCI uses CutOne® reaction buffer and is compatible with double digestion with other LightNing® restriction enzymes. However, it is advisable to avoid extended digestion (exceeding 3 hours) to prevent star activity.
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/25516.pdf
Concentration
2 U/μL
Components
• BbvCI (2 U/μl) : 25 μl • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml
Features & Benefits
Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 37°C; Refer to "Protocol for DNA Digestion" for reaction setup. Heat Inactivation Incubation at 80°C for 20 minutes. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Shipping Conditions
2~8°C
Storage Conditions
Store at -20°C for 2 years.
Shelf Life
2 Years
Cut Site
CCTCAGC 5'...C C↓T C A G C...3' 3'...G G A G T↑C G ...5'

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