LightNing® Tsp45I

CAT: 1465-EG24517SSize: 25 ReactionsDry Ice: NoHazardous: No
CAT#:1465-EG24517SSize:25 Reactions
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/04xianzhimei/EG24517.pdf
Concentration
10 U/μL
Components
• LightNing® Tsp45I (10 U/μl) : 25 μl (250 U) • 10x CutOne® Buffer: 1 ml • 10x CutOne® Color Buffer: 1 ml
Features & Benefits
The reaction can be completed within 15 minutes Recommended Reaction Conditions 1x CutOne® Buffer; Incubate at 65°C; Refer to "Protocol for Fast DNA Digestion" for reaction setup. Heat Inactivation This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit. Methylation sensitivity Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.
Shipping Conditions
2~8°C
Storage Conditions
-20°C
Shelf Life
2 Years
Isoschizomers
NmuCI, TseFI
Cut Site
GTSAC 5'...↓G T S A C...3' 3'... C A S T G↑...5'

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