M-MLV GIV Reverse Transcriptase

CAT: 1465-EG24101SSize: 10000 UDry Ice: NoHazardous: No
CAT#:1465-EG24101SSize:10000 U
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
M-MLV GIV Reverse Transcriptase is a fourth-generation M-MLV reverse transcriptase obtained through genetic modification and recombination technologies. Compared with the wild-type M-MLV reverse transcriptase, it lacks RNase H activity. Key enhancements include superior thermal stability, with an optimal reaction temperature of 55°C and sustained 50% activity at 70°C. Furthermore, it demonstrates improved tolerance to complex secondary structures , accelerated reaction speed (completing reactions in as little as 10 minutes), and high template affinity. These combined properties ensure robust performance with low-copy templates and enable efficient synthesis of full-length cDNA fragments up to 20 kb.
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/02nizhuanlu/EG24101.pdf
Concentration
200 U/μL
Components
• M-MLV GIV Reverse Transcriptase (200 U/μl) : 50 μl • 5x M-MLV First Strand Buffer: 500 μl • 0.1 M DTT: 100 μl
Shipping Conditions
2~8°C
Storage Conditions
Store at -20°C for 2 years.
Shelf Life
2 Years

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