MMP13 (Cleaved-Tyr104) rabbit pAb

• Background:

  Cofactor:Binds 2 zinc ions per subunit., cofactor:Binds 4 calcium ions per subunit., disease:Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common., domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme., function:Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process., similarity:Belongs to the peptidase M10A family., similarity:Contains 4 hemopexin-like domains., tissue specificity:Seems to be specific to breast carcinomas.

• Description:

  Cofactor: Binds 2 zinc ions per subunit. Cofactor: Binds 4 calcium ions per subunit. Disease: Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM: 602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common. Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. function: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. similarity: Belongs to the peptidase M10A family. similarity: Contains 4 hemopexin-like domains. tissue specificity: Seems to be specific to breast carcinomas.

• Synonyms:

  Collagenase 3 (EC 3.4.24.-;Matrix metalloproteinase-13;MMP-13)

• Gene ID:

  4322

• UniProt:

  P45452

• Cellular Locus:

  Secreted, extracellular space, extracellular matrix. Secreted.

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat, Mouse,

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Synthesized peptide derived from human MMP13 (Cleaved-Tyr104)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, ELISA, IHC

• Validated Applications:

  WB, ELISA, IHC

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

• Molecular Weight:

  42 53kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  42 - 53 kD

• Subcellular Location:

  Secreted, extracellular space, extracellular matrix . Secreted .

• Other Product Names:

  Collagenase 3 (EC 3.4.24.-; Matrix metalloproteinase-13; MMP-13)

• Gene ID (Human):

  4322

• SwissProt (Human):

  P45452