Crystal Violet Staining Solution

CAT: 0931-C0160Size: 100 mLDry Ice: NoHazardous: No
CAT#:0931-C0160Size:100 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Purity
0.996
Bioactivity
Crystal violet is a basic triphenylmethane dye. Crystal violet staining solution is commonly used in Gram staining to distinguish between Gram-positive and Gram-negative bacteria. Crystal violet can bind to the peptidoglycan in bacterial cell walls, forming a colored complex. Because Gram-positive bacteria have a thicker cell wall with a high peptidoglycan content, the crystal violet-iodine complex is difficult to wash out with ethanol, so they appear purple. In contrast, Gram-negative bacteria have thinner cell walls with less peptidoglycan, allowing the crystal violet to be removed by ethanol; after counterstaining, they appear red. In addition, crystal violet can bind to nucleic acids and acidic substances within cells to form a purple complex, thereby staining cells for visualization of colony numbers. This makes it useful in cytological experiments for assessing cell proliferation or migration activity.
Shipping Conditions
Cool pack
Storage Temperature
-20°C

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