Annexin V-APC

• Description:

  Annexin A5 (or Annexin V) is a cellular protein inthe annexin group. Annexin A5 is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The annexin A5 affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (annexin V binding to PS is Ca2+ dependent) . The assay combines annexin V staining of PS membrane events with the staining of cell nucleus with PI or AAD-7 in living cells to distinguish from dead cells.

• Applications:

  Flow cytometry (Use 5μl for 105cells) It may can work in Immunofluorescence.

• Buffer:

  In PBS (pH7.2), 0.09% NaN3

• Storage Conditions:

  Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.