Polyclonal Anti-PCNA

• Background :

  Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. And It has to be loaded onto DNA in order to be able to stimulate APEX2.

• Host :

  Rabbit

• Reactivity :

  Human, mouse, rat, monkey, Chinese hamster, dog

• Immunogen :

  A synthetic peptide corresponding to internal residues of human PCNA.

• Applications :

  Western Blot (1:5000-20000)

• Form :

  Liquid

• References & Citations :

  1. Bowman GD, O'Donnell M, Kuriyan J (2004) . "Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex". Nature 429 (6993) : 724–730. 2. Burkovics P., Hajdu I., Szukacsov V., Unk I., Haracska L."Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage." Nucleic Acids Res. 37:4247-4255 (2009) 3. Motegi A., Liaw H.-J., Lee K.-Y., Roest H.P., Maas A., Wu X., Moinova H., Markowitz S.D., Ding H., Hoeijmakers J.H.J., Myung K. "Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks." Proc. Natl. Acad. Sci. U.S.A. 105:12411-12416 (2008)

• Storage Conditions :

  Store at -20°C for 1 year. Avoid repeated freeze and thaw cycles.

• Specificity :

  Detect the endogenous levels of total PCNA protein.