β-Lactamase coupling antigen

• Background:

  Beta-lactamases are enzymes (EC 3.5.2.6) produced by bacteria that provide multi- resistance to β-lactam antibiotics such as penicillins, cephalosporins, cephamycins, and carbapenems (ertapenem), although carbapenems are relatively resistant to beta-lactamase. Beta-lactamase provides antibiotic resistance by breaking the antibiotics' structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a β-lactam. Through hydrolysis, the enzyme lactamase breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.Beta-lactam antibiotics are typically used to treat a broad spectrum of Gram-positive and Gram-negative bacteria.Beta-lactamases produced by Gram-negative organisms are usually secreted, especially when antibiotics are present in the environment.

• Applications:

  ELISA; Colloidal gold

• Purity:

  >95% by HPLC & SDS-PAGE

• Form:

  Liquid

• Buffer:

  Protein A or G purified and supplied in 0.01 M PBS (pH7.4)

• References & Citations:

  1. Neu HC (June 1969) . "Effect of beta-lactamase location in Escherichia coli on penicillin synergy". Applied Microbiology. 17 (6) : 783–6.

• Storage Conditions:

  Aliquot and store at -20°C for 2 years. Avoid repeated freeze and thaw cycles.