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Recombinant mouse PRMT1 protein

CAT: 0112-PRM0801-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-PRM0801-100Size:100 µg
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Description
Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Mouse PRMT1 (AAF37293) shares 99. 9% sequence identity with the human proteins (NP_938074) . Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 30 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Product Name Alternative
Protein arginine N-methyltransferase 1 isoform 2, Histone-arginine N-methyltransferase PRMT1, Heterogeneous nuclear ribonucleoproteins methyltransferase-like 2, Hrmt1l2, Mrmt1
Expression System
E.coli
Antigen Species
Mouse
Tag
His-MBP-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 30 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Molecular Weight
84 kDa (750aa)
Additionnal Information
R1B4, Protein arginine N-methytransferase 1, Protein arginine N-methyltransferase 1 isoform 2, Protein arginine N-methyltransferase 1 ANM 1, Protein arginine N-methyltransferase 1, Protein arginine N methyltransferase1, Protein arginine N methyltransferase 1, PRMT1 isoform2, PRMT1, PRMT 1, Mrmt1, Mrmt 1, IR1B 4, IR1 B4, Interferon receptor 1bound protein 4, Interferon receptor 1 bound protein4, Interferon receptor 1 bound protein 4, Human mRNA for suppressor for yeast mutant complete cds, Human mRNA for suppressor for yeast mutant, Hrmt1l2, HRMT1L 2, Hrmt112, HRMT1 L2, HMT2, HMT1 hnRNP methyltransferase like 2, HMT1 hnRNP methyltransferase, HMT 2, Histone-arginine N-methyltransferase PRMT1, Heterogeneous nuclear ribonucleoproteins methyltransferase-like 2, Heterogeneous nuclear ribonucleoproteins methyltransferase like2, Heterogeneous nuclear ribonucleoproteins methyltransferase like 2, HCP 1, AW214366, "S, cerevisiae like 2", "HMT1 hnRNP methyltransferase like 2 (S, cerevisiae) ", "HMT
References & Citations
Herrmann F., et al. (2005) . J Biol Chem. 280 (45) :38005-10.; ; Abramovich C., et al. (1997) . EMBO J. 16 (2) :260-6. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 40 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 4mM MgCl2, 2mM DTT, 40% glycerol
Scientific Category
Histone Related
NCBI Accession Number
NP_001239405.1
Uniprot Accession Number
Q9JIF0
Species
Mouse
AA Sequence
MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

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