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Recombinant human MGMT protein

CAT: 0112-MMT0901-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-MMT0901-500Size:500 µg
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Description
O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.
Product Name Alternative
O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
23.8 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
MGMT, O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase., MMT0901-10 µg, MMT0901-20 µg, MMT0901-50 µg, MMT0901-100 µg, MMT0901-250 µg, MMT0901-500 µg, MMT0901-1 mg, MMT0901-10, MMT0901-20, MMT0901-50, MMT0901-100, MMT0901-250, MMT0901-500, MMT0901-1
References & Citations
Matsukura S., et al. (2001) Annals of Surgical Oncology, 8 (10) :807--816; ; Esteller M., et al. (2002) J Natl Cancer Inst. 2, 26-32.;
Other References
Hongo A, et al. A radioisotope-nondependent high-sensitivity method for measuring the activity of glioblastoma-related O (6) -methylguanine DNA methyltransferase. (Anal Biochem. 2015) {https://pubmed.ncbi.nlm.nih.gov/25173514/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002403.3
Uniprot Accession Number
P16455
Species
Human
AA Sequence
MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN

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