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Recombinant human IL-4 protein

CAT: 0112-ILD0905-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ILD0905-010Size:10 µg
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Description
Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naive helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.
Specifications
The ED50 for this effect is less or equal to 0.5 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Product Name Alternative
Pitrakinra, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin 4, IL-4, BSF1, Binetrakin, BCGF1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
The ED50 for this effect is less or equal to 0.5 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Molecular Weight
17.2 kDa (150aa) confirmed by MALDI-TOF
Additionnal Information
Pitrakinra, Pitrakinara, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin-4, Interleukin4, Interleukin 4 isoform 1, Interleukin 4, IL-4, IL4, IL 4, BSF-1, BSF1, BSF 1, Binetrakin, BCGF-1, BCGF1, BCGF 1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF, B cell stimulatory factor 1, B cell growth factor 1, ILD0905-10 µg, ILD0905-20 µg, ILD0905-50 µg, ILD0905-100 µg, ILD0905-250 µg, ILD0905-500 µg, ILD0905-1 mg, ILD0905-010, ILD0905-020, ILD0905-050, ILD0905-100, ILD0905-250, ILD0905-500, ILD0905-01M
References & Citations
Van Kimmenade A., et al. (1988) Eur J Biochem. 173 (1) :109-14.; ; Yokota T., et al. (1986) Proc. Natl. Acad. Sci. uSA. 83 (16) :5894-8.;
Other References
Lee HM, et al. A comparative study of the effects of inhibitory cytokines on human natural killer cells and the mechanistic features of transforming growth factor-beta. (Cell Immunol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24879062/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000580
Uniprot Accession Number
P05112
Species
Human
AA Sequence
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

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