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Human VAMP-2 antibody

CAT: 0112-AVA0710-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-AVA0710-050Size:50 µL
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Description
Synaptobrevin 2 (Vesicle-associated membrane, VAMP2), which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal tail. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. This antibody recognizes specifically synaptobrevin 2, but it also shows a low affinity to synaptobrevin 1.
Product Name Alternative
Vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2)
Host
Mouse
Antigen Species
Human
Reactivity
Human, Mouse
Immunogen
Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
300000
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VAMP2, AVA0710-10 µg, AVA0710-20 µg, AVA0710-50 µg, AVA0710-100 µg, AVA0710-250 µg, AVA0710-500 µg, AVA0710-1 mg, AVA0710-10, AVA0710-20, AVA0710-50, AVA0710-100, AVA0710-250, AVA0710-500, AVA0710-1
References & Citations
Andres C et al., (1995) Proc Natl Acad Sci USA 92:5987-5991.; ; Jacobsson G et al., (1998) European Journal of Neuroscience 16:301-316.; ; Scales SJ. et al (2002) J Biol. Chem. 227 (31) :28271-28279.; ; Pennuto M et al., (2003) Mol Biol Cell. 14:4909-19.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_055047
Uniprot Accession Number
P63027
WB Description
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human VAMP1 protein; ; Lane 2.: Recombinant human VAMP2 protein; ; Lane 3.: Recombinant human VAMP3 protein; ; The tissue lysate (50 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain Tissue lysate{AVA0710-WB2.jpg}The tissue lysate (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Rat brain Tissue lysate{AVA0710-WB3.jpg}
IF Description
ICC/IF analysis of VAMP-2 in U87MG cells. The cell was stained with AVA0710 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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