Recombinant human FLRT3 protein

• Description:

  FLRT3, also known as leucine-rich repeat transmembrane protein, is a member of the fibronectin leucine rich transmembrane protein (FLRT) family. It contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain. And It expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart. The members of the FLRT family may have a function in cell adhesion and/or receptor signaling. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development. The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association. Also, It may have a crucial role in regulating cellular adhesion between the epithelial apical ridge and the underlying mesenchyme and in establishing the dorso-ventral position of the ridge. Recombinant human FLRT3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

• Specifications:

  Human FLRT3 stimulates cell adhesion of the Neuro-2a mouse neuroblast cells. This effect is more to 40%.

• Product Name Alternative:

  Fibronectin leucine rich transmembrane protein 3, HH21, leucine-rich repeat transmembrane protein FLRT3, Fibronectin-like domain-containing leucine-rich transmembrane protein 3

• Expression System:

  HEK293

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE, Bioactivity

• Concentration:

  0.25 mg/mL (determined by absorbance at 280 nm)

• Endotoxin:

  < 1 EU per 1ug of protein (determined by LAL method)

• Purity:

  > 95% by SDS-PAGE

• Bioactivity:

  Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a neuroblast cells. When cells are added to human FLRT3 coated plates 5 µg/mL. This effect is more to 40%.

• Molecular Weight:

  57.3 kDa (506aa)

• Additionnal Information:

  ATGP4132-10 µg, ATGP4132-20 µg, ATGP4132-50 µg, ATGP4132-100 µg, ATGP4132-250 µg, ATGP4132-500 µg, ATGP4132-1 mg, ATGP4132-010, ATGP4132-020, ATGP4132-050, ATGP4132-100, ATGP4132-250, ATGP4132-500, ATGP4132-01M

• References & Citations:

  Lacy S E., et al. (1999) Genomics. 62:417-426.; ; Haines B. P., et al. (2006) Dev. Biol. 297:14-25.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

• Scientific Category:

  Signal Transduction, Cytoskeleton, Adhesion & Movement

• NCBI Accession Number:

  NP_037413.1

• Uniprot Accession Number:

  Q9NZU0

• Species:

  Human

• N Terminal Sequence:

  Lys20