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Recombinant mouse PKM protein

CAT: 0112-ATGP3610-004Size: 4 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3610-004Size:4 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Pkm, also known as pyruvate kinase PKM isoform M2, is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. Pkm interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant mouse Pkm, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C
Product Name Alternative
Pyruvate kinase PKM isoform M2, Pyruvate kinase muscle isozyme, pyruvate kinase M1/2, Threonine-protein kinase PKM2, Tyrosine-protein kinase PKM2, Pk3, Pkm2, Pykm
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 85% by SDS-PAGE
Bioactivity
Specific activity: > 50,000pmol/min/ug. One unit will convert 1.0pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C
Molecular Weight
60.2 kDa (554aa) Confirmed by MALDI-TOF
Additionnal Information
Pkm, Pyruvate kinase PKM isoform M2, AA414905, AL024370, AL024424, Pk-2, Pk-3, Pk3, Pkm2, ATGP3610-10 µg, ATGP3610-20 µg, ATGP3610-50 µg, ATGP3610-100 µg, ATGP3610-250 µg, ATGP3610-500 µg, ATGP3610-1 mg, ATGP3610-10, ATGP3610-20, ATGP3610-50, ATGP3610-100, ATGP3610-250, ATGP3610-500, ATGP3610-1
References & Citations
Spoden GA., et al. (2009) . Exp Cell Res. 315 (16) :2765-74; ; Mazurek S., et al. (2007) . Ernst Schering Found Symp Proc. (4) :99-124.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_035229
Uniprot Accession Number
P52480
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP

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