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Recombinant human CSTF1 protein

CAT: 0112-ATGP3573-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3573-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CSTF1, also known as cleavage stimulation factor subunit 1, is one of the multiple factors required for polyadenylation of mammalian pre-mRNAs in vitro. It is functioning with cleavage-polyadenylation specificity factor, two cleavage factors, and poly (A) + polymerase. It is involved in the 3' end processing of messenger RNA precursors. Recombinant human CSTF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Name Alternative
Cleavage stimulation factor subunit 1, CSTF1, CstF-50, CstFp50
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
49.1 kDa (437aa)
Additionnal Information
CSTF1, Cleavage stimulation factor subunit 1, CSTF1, CstF-50, CstFp50, ATGP3573-10 µg, ATGP3573-20 µg, ATGP3573-50 µg, ATGP3573-100 µg, ATGP3573-250 µg, ATGP3573-500 µg, ATGP3573-1 mg, ATGP3573-10, ATGP3573-20, ATGP3573-50, ATGP3573-100, ATGP3573-250, ATGP3573-500, ATGP3573-1
References & Citations
Takagaki Y., et al. (1994) Nature. 372:471-474.; ; Ruegsegger U., et al. (1996) J Biol Chem. 271:6107-6113.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001028693
Uniprot Accession Number
Q05048
Species
Human
N Terminal Sequence
Met1
AA Sequence
MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH

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