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Recombinant human PGP protein

CAT: 0112-ATGP3564-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3564-100Size:100 µg
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Description
Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level) . The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Product Name Alternative
Phosphoglycolate phosphatase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 3,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Molecular Weight
36.5 kDa (345aa) confirmed by MALDI-TOF
Additionnal Information
PGP, Phosphoglycolate phosphatase, ATGP3564-10 µg, ATGP3564-20 µg, ATGP3564-50 µg, ATGP3564-100 µg, ATGP3564-250 µg, ATGP3564-500 µg, ATGP3564-1 mg, ATGP3564-10, ATGP3564-20, ATGP3564-50, ATGP3564-100, ATGP3564-250, ATGP3564-500, ATGP3564-1
References & Citations
Barker R.F., et al. (1978) Ann. Hum. Genet. 42:143-151
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001035830
Uniprot Accession Number
A6NDG6
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG

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