Recombinant human NARS protein

• Description:

  NARS also known as asparagine--tRNA ligase, cytoplasmic. This protein acts as an enzyme. It is known to catalyze the following reaction: ATP + L-asparagine + tRNA (Asn) = AMP + diphosphate + L-asparaginyl-tRNA (Asn) . Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. Recombinant human NARS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

• Product Name Alternative:

  Asparagine--tRNA ligase cytoplasmic, ASNRS, NARS1

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  0.25 mg/mL (determined by absorbance at 280 nm)

• Purity:

  > 85% by SDS-PAGE

• Molecular Weight:

  65.3 kDa (571aa)

• Additionnal Information:

  NARS, Asparagine--tRNA ligase cytoplasmic, ASNRS, NARS1, ATGP3157-10 µg, ATGP3157-20 µg, ATGP3157-50 µg, ATGP3157-100 µg, ATGP3157-250 µg, ATGP3157-500 µg, ATGP3157-1 mg, ATGP3157-10, ATGP3157-20, ATGP3157-50, ATGP3157-100, ATGP3157-250, ATGP3157-500, ATGP3157-1

• References & Citations:

  Beaulande M., et al. (1998) Nucleic Acids. 26 (2) :521-4.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

• Scientific Category:

  Epigenetics and Nuclear Signaling

• NCBI Accession Number:

  NP_004530

• Uniprot Accession Number:

  O43776

• Species:

  Human

• AA Sequence:

  MVLAELY VSDREGSDAT GDGTKEKPFK TGLKALMTVG KEPFPTIYVD SQKENERWNV ISKSQLKNIK KMWHREQMKS ESREKKEAED SLRREKNLEE AKKITIKNDP SLPEPKCVKI GALEGYRGQR VKVFGWVHRL RRQGKNLMFL VLRDGTGYLQ CVLADELCQC YNGVLLSTES SVAVYGMLNL TPKGKQAPGG HELSCDFWEL IGLAPAGGAD NLINEESDVD VQLNNRHMMI RGENMSKILK ARSMVTRCFR DHFFDRGYYE VTPPTLVQTQ VEGGATLFKL DYFGEEAFLT QSSQLYLETC LPALGDVFCI AQSYRAEQSR TRRHLAEYTH VEAECPFLTF DDLLNRLEDL VCDVVDRILK SPAGSIVHEL NPNFQPPKRP FKRMNYSDAI VWLKEHDVKK EDGTFYEFGE DIPEAPERLM TDTINEPILL CRFPVEIKSF YMQRCPEDSR LTESVDVLMP NVGEIVGGSM RIFDSEEILA GYKREGIDPT PYYWYTDQRK YGTCPHGGYG LGLERFLTWI LNRYHIRDVC LYPRFVQRCT P