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Recombinant human NARS protein

CAT: 0112-ATGP3157-004Size: 4 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3157-004Size:4 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NARS also known as asparagine--tRNA ligase, cytoplasmic. This protein acts as an enzyme. It is known to catalyze the following reaction: ATP + L-asparagine + tRNA (Asn) = AMP + diphosphate + L-asparaginyl-tRNA (Asn) . Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. Recombinant human NARS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Name Alternative
Asparagine--tRNA ligase cytoplasmic, ASNRS, NARS1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Purity
> 85% by SDS-PAGE
Molecular Weight
65.3 kDa (571aa)
Additionnal Information
NARS, Asparagine--tRNA ligase cytoplasmic, ASNRS, NARS1, ATGP3157-10 µg, ATGP3157-20 µg, ATGP3157-50 µg, ATGP3157-100 µg, ATGP3157-250 µg, ATGP3157-500 µg, ATGP3157-1 mg, ATGP3157-10, ATGP3157-20, ATGP3157-50, ATGP3157-100, ATGP3157-250, ATGP3157-500, ATGP3157-1
References & Citations
Beaulande M., et al. (1998) Nucleic Acids. 26 (2) :521-4.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
NP_004530
Uniprot Accession Number
O43776
Species
Human
AA Sequence
MVLAELY VSDREGSDAT GDGTKEKPFK TGLKALMTVG KEPFPTIYVD SQKENERWNV ISKSQLKNIK KMWHREQMKS ESREKKEAED SLRREKNLEE AKKITIKNDP SLPEPKCVKI GALEGYRGQR VKVFGWVHRL RRQGKNLMFL VLRDGTGYLQ CVLADELCQC YNGVLLSTES SVAVYGMLNL TPKGKQAPGG HELSCDFWEL IGLAPAGGAD NLINEESDVD VQLNNRHMMI RGENMSKILK ARSMVTRCFR DHFFDRGYYE VTPPTLVQTQ VEGGATLFKL DYFGEEAFLT QSSQLYLETC LPALGDVFCI AQSYRAEQSR TRRHLAEYTH VEAECPFLTF DDLLNRLEDL VCDVVDRILK SPAGSIVHEL NPNFQPPKRP FKRMNYSDAI VWLKEHDVKK EDGTFYEFGE DIPEAPERLM TDTINEPILL CRFPVEIKSF YMQRCPEDSR LTESVDVLMP NVGEIVGGSM RIFDSEEILA GYKREGIDPT PYYWYTDQRK YGTCPHGGYG LGLERFLTWI LNRYHIRDVC LYPRFVQRCT P

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