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Recombinant human PYCR2 protein

CAT: 0112-ATGP3066-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3066-100Size:100 µg
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Description
PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
36 kDa (343aa) confirmed by MALDI-TOF
Additionnal Information
PYCR2, Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2, ATGP3066-10 µg, ATGP3066-20 µg, ATGP3066-50 µg, ATGP3066-100 µg, ATGP3066-250 µg, ATGP3066-500 µg, ATGP3066-1 mg, ATGP3066-10, ATGP3066-20, ATGP3066-50, ATGP3066-100, ATGP3066-250, ATGP3066-500, ATGP3066-1
References & Citations
De Ingeniis J, et al. (2012) . PLoS One. 7 (9) :e45190.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 5mM DTT, 1mM EDTA
Scientific Category
Metabolism
NCBI Accession Number
NP_037460
Uniprot Accession Number
Q96C36
Species
Human
AA Sequence
MSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRK MGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKV IRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDA LADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINA VEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD