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Recombinant human NMT1 protein

CAT: 0112-ATGP2932-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2932-500Size:500 µg
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Description
Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO. Recombinant human NMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
59.2 kDa (519aa)
Additionnal Information
NMT1, Glycylpeptide N-tetradecanoyltransferase 1, Glycylpeptide N-tetradecanoyltransferase 1, NMT, Myristoyl-CoA:protein N-myristoyltransferase 1, ATGP2932-10 µg, ATGP2932-20 µg, ATGP2932-50 µg, ATGP2932-100 µg, ATGP2932-250 µg, ATGP2932-500 µg, ATGP2932-1 mg, ATGP2932-10, ATGP2932-20, ATGP2932-50, ATGP2932-100, ATGP2932-250, ATGP2932-500, ATGP2932-1
References & Citations
Glover C.J. et al. (1997) J. Biol. Chem. 272:28680-28689.; ; Van Damme P. et al. (2012) Proc. Natl. Acad. Sci. u.S.A. 109:12449-12454.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Cancer
NCBI Accession Number
NP_066565.1
Uniprot Accession Number
P30419
Species
Human
AA Sequence
MADESET AVKPPAPPLP QMMEGNGNGH EHCSDCENEE DNSYNRGGLS PANDTGAKKK KKKQKKKKEK GSETDSAQDQ PVKMNSLPAE RIQEIQKAIE LFSVGQGPAK TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT WDALDLGDRG VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL PQWHCGVRVV SSRKLVGFIS AIPANIHIYD TEKKMVEINF LCVHKKLRSK RVAPVLIREI TRRVHLEGIF QAVYTAGVVL PKPVGTCRYW HRSLNPRKLI EVKFSHLSRN MTMQRTMKLY RLPETPKTAG LRPMETKDIP VVHQLLTRYL KQFHLTPVMS QEEVEHWFYP QENIIDTFVV ENANGEVTDF LSFYTLPSTI MNHPTHKSLK AAYSFYNVHT QTPLLDLMSD ALVLAKMKGF DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWKCPSMG AEKVGLVLQ

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