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Recombinant human RTCA protein

CAT: 0112-ATGP2735-004Size: 4 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2735-004Size:4 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RTCA catalyzes the conversion of 3'-phosphate to a 2', 3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
RNA 3-terminal phosphate cyclase isoform b, RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
41.7 kDa (389aa) confirmed by MALDI-TOF
Additionnal Information
RTCA, RNA 3-terminal phosphate cyclase isoform b, RNA 3'-terminal phosphate cyclase, RPC, RTC1, RTCD1, ATGP2735-10 µg, ATGP2735-20 µg, ATGP2735-50 µg, ATGP2735-100 µg, ATGP2735-250 µg, ATGP2735-500 µg, ATGP2735-1 mg, ATGP2735-10, ATGP2735-20, ATGP2735-50, ATGP2735-100, ATGP2735-250, ATGP2735-500, ATGP2735-1
References & Citations
Genschik P., Billy E., et al. (1997) EMBO J. 16:2955-2967
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003720.1
Uniprot Accession Number
O00442
Species
Human
AA Sequence
MAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL

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