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Recombinant human GET4 protein

CAT: 0112-ATGP2559-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2559-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GET4 is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. Recombinant human GET4 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Name Alternative
Golgi to ER traffic protein 4 homolog, Golgi to ER traffic protein 4 homolog (S. cerevisiae), C7orf20, CEE, CGI-20, TRC35
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.9 kDa (350aa)
Additionnal Information
GET4, Golgi to ER traffic protein 4 homolog, Golgi to ER traffic protein 4 homolog (S. cerevisiae), C7orf20, CEE, CGI-20, TRC35, ATGP2559-10 µg, ATGP2559-20 µg, ATGP2559-50 µg, ATGP2559-100 µg, ATGP2559-250 µg, ATGP2559-500 µg, ATGP2559-1 mg, ATGP2559-10, ATGP2559-20, ATGP2559-50, ATGP2559-100, ATGP2559-250, ATGP2559-500, ATGP2559-1
References & Citations
Mariappan M., Li X., et al. (2010) Nature 466:1120-1124.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Scientific Category
Other
NCBI Accession Number
NP_057033
Uniprot Accession Number
Q7L5D6
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLCLK NKSSASVVFT TYTQKHPSIE DGPPFVEPLL NFIWFLLLAV DGGKLTVFTV LCEQYQPSLR RDPMYNEYLD RIGQLFFGVP PKQTSSYGGL LGNLLTSLMG SSEQEDGEES PSDGSPIELD

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