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Recombinant human EED protein

CAT: 0112-ATGP2154-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2154-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta 7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Recombinant EED protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Polycomb protein EED isoform a, embryonic ectoderm development, HEED; WAIT1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
52.6 kDa (464aa)
Additionnal Information
EED, ATGP2154-10 µg, ATGP2154-20 µg, ATGP2154-50 µg, ATGP2154-100 µg, ATGP2154-250 µg, ATGP2154-500 µg, ATGP2154-1 mg, ATGP2154-10, ATGP2154-20, ATGP2154-50, ATGP2154-100, ATGP2154-250, ATGP2154-500, ATGP2154-1
References & Citations
Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (Nov 1998) . J Biol Chem 273 (42) : 27459-66; ; Schumacher A, Lichtarge O, Schwartz S, Magnuson T (Jan 1999) . Genomics 54 (1) : 79-88
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003788.2
Uniprot Accession Number
O75530
Species
Human
AA Sequence
MSEREVS TAPAGTDMPA AKKQKLSSDE NSNPDLSGDE NDDAVSIESG TNTERPDTPT NTPNAPGRKS WGKGKWKSKK CKYSFKCVNS LKEDHNQPLF GVQFNWHSKE GDPLVFATVG SNRVTLYECH SQGEIRLLQS YVDADADENF YTCAWTYDSN TSHPLLAVAG SRGIIRIINP ITMQCIKHYV GHGNAINELK FHPRDPNLLL SVSKDHALRL WNIQTDTLVA IFGGVEGHRD EVLSADYDLL GEKIMSCGMD HSLKLWRINS KRMMNAIKES YDYNPNKTNR PFISQKIHFP DFSTRDIHRN YVDCVRWLGD LILSKSCENA IVCWKPGKME DDIDKIKPSE SNVTILGRFD YSQCDIWYMR FSMDFWQKML ALGNQVGKLY VWDLEVEDPH KAKCTTLTHH KCGAAIRQTS FSRDSSILIA VCDDASIWRW DRLR

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