Recombinant human CD74 protein

• Description:

  HLA class II histocompatibility antigen gamma chain isoform b, also known as CD74, is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. It is upregulated in several cancers and is also expressed by nonimmune cells during inflammation. Recombinant human CD74 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative:

  HLA class II histocompatibility antigen gamma chain isoform b, DHLAG, HLADG, Ia-GAMMA, II

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  0.5 mg/mL (determined by Bradford assay)

• Endotoxin:

  < 1 EU per 1ug of protein (determined by LAL method)

• Purity:

  > 90% by SDS-PAGE

• Molecular Weight:

  20.6 kDa (183aa) confirmed by MALDI-TOF

• Additionnal Information:

  CD74, ATGP2104-10 µg, ATGP2104-20 µg, ATGP2104-50 µg, ATGP2104-100 µg, ATGP2104-250 µg, ATGP2104-500 µg, ATGP2104-1 mg, ATGP2104-10, ATGP2104-20, ATGP2104-50, ATGP2104-100, ATGP2104-250, ATGP2104-500, ATGP2104-1

• References & Citations:

  Wilson K M., et al. (1993) Immunology. 79:331-335; ; Henne C., et al. (1995) Immunology. 84:177-182.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT

• Scientific Category:

  Immunology

• NCBI Accession Number:

  NP_004346.1

• Uniprot Accession Number:

  P04233

• Species:

  Human

• AA Sequence:

  QQQGRLD KLTVTSQNLQ LENLRMKLPK PPKPVSKMRM ATPLLMQALP MGALPQGPMQ NATKYGNMTE DHVMHLLQNA DPLKVYPPLK GSFPENLRHL KNTMETIDWK VFESWMHHWL LFEMSRHSLE QKPTDAPPKE SLELEDPSSG LGVTKQDLGP VPM