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Recombinant human U2AF1 protein

CAT: 0112-ATGP2095-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2095-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
U2AF1 belongs to the splicing factor SR family of genes. u2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of u2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Recombinant human u2AF1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Name Alternative
U2 small nuclear RNA auxiliary factor 1, FP793, RN, RNu2AF1, Splicing factor u2AF 35 kDa subunit, u2 auxiliary factor 35 kDa subunit, RN, RNu2AF1, u2AF35, u2AFBP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30.3 kDa (263aa)
Additionnal Information
U2AF1, u2 small nuclear RNA auxiliary factor 1, FP793, RN, RNu2AF1, Splicing factor u2AF 35 kDa subunit, u2 auxiliary factor 35 kDa subunit, RN, RNu2AF1, u2AF35, u2AFBP, ATGP2095-10 µg, ATGP2095-20 µg, ATGP2095-50 µg, ATGP2095-100 µg, ATGP2095-250 µg, ATGP2095-500 µg, ATGP2095-1 mg, ATGP2095-10, ATGP2095-20, ATGP2095-50, ATGP2095-100, ATGP2095-250, ATGP2095-500, ATGP2095-1
References & Citations
Thol, F., et al. (2012) Blood 119 (15), 3578-3584; ; Makishima, H., et al. (2012) Blood 119 (14), 3203-3210
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001020374
Uniprot Accession Number
Q01081
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF

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