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Recombinant human MIS12 protein

CAT: 0112-ATGP1928-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1928-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Name Alternative
Protein MIS12 homolog, hMis12, KNTC2AP, MTW1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.5 kDa (228aa)
Additionnal Information
MIS12, Protein MIS12 homolog, hMis12, KNTC2AP, MTW1, ATGP1928-10 µg, ATGP1928-20 µg, ATGP1928-50 µg, ATGP1928-100 µg, ATGP1928-250 µg, ATGP1928-500 µg, ATGP1928-1 mg, ATGP1928-10, ATGP1928-20, ATGP1928-50, ATGP1928-100, ATGP1928-250, ATGP1928-500, ATGP1928-1
References & Citations
Kline S L., et al. (2006) J Cell Biol. 173:9-17.; ; Goshima G., et al. (2003) J Cell Biol. 160:25-39.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_076944
Uniprot Accession Number
Q9H081
Species
Human
AA Sequence
MSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS

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