Recombinant human CD1b protein

CAT: 0112-ATGP1783-020Size: 20 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP1783-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. This protein contains 1 Ig-like (immunoglobulin-like) domain. CD1B is antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. Recombinant human CD1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Name Alternative
T-cell surface glycoprotein CD1b, CD1, CD1A, R1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.2 kDa (310aa) confirmed by MALDI-TOF
Additionnal Information
CD1b, T-cell surface glycoprotein CD1b, CD1, CD1A, R1, ATGP1783-10 µg, ATGP1783-20 µg, ATGP1783-50 µg, ATGP1783-100 µg, ATGP1783-250 µg, ATGP1783-500 µg, ATGP1783-1 mg, ATGP1783-10, ATGP1783-20, ATGP1783-50, ATGP1783-100, ATGP1783-250, ATGP1783-500, ATGP1783-1
References & Citations
Shamshiev A., et al. (2000) Immunity 13:255-264 ; ; Winau F., et al. (2004) Nat. Immunol. 5:169-174;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol,1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_001755
Uniprot Accession Number
P29016
Species
Human
AA Sequence
SEHAFQ GPTSFHVIQT SSFTNSTWAQ TQGSGWLDDL QIHGWDSDSG TAIFLKPWSK GNFSDKEVAE LEEIFRVYIF GFAREVQDFA GDFQMKYPFE IQGIAGCELH SGGAIVSFLR GALGGLDFLS VKNASCVPSP EGGSRAQKFC ALIIQYQGIM ETVRILLYET CPRYLLGVLN AGKADLQRQV KPEAWLSSGP SPGPGRLQLV CHVSGFYPKP VWVMWMRGEQ EQQGTQLGDI LPNANWTWYL RATLDVADGE AAGLSCRVKH SSLEGQDIIL YWRNPTSIGS

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