Recombinant human CD1b protein

• Description :

  CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. This protein contains 1 Ig-like (immunoglobulin-like) domain. CD1B is antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. Recombinant human CD1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

• Product Name Alternative :

  T-cell surface glycoprotein CD1b, CD1, CD1A, R1

• Expression System :

  E.coli

• Antigen Species :

  Human

• Tag :

  His-Tag

• Applications :

  SDS-PAGE

• Concentration :

  0.5 mg/mL (determined by Bradford assay)

• Purity :

  > 90% by SDS-PAGE

• Molecular Weight :

  34.2 kDa (310aa) confirmed by MALDI-TOF

• Additionnal Information :

  CD1b, T-cell surface glycoprotein CD1b, CD1, CD1A, R1, ATGP1783-10 µg, ATGP1783-20 µg, ATGP1783-50 µg, ATGP1783-100 µg, ATGP1783-250 µg, ATGP1783-500 µg, ATGP1783-1 mg, ATGP1783-10, ATGP1783-20, ATGP1783-50, ATGP1783-100, ATGP1783-250, ATGP1783-500, ATGP1783-1

• References & Citations :

  Shamshiev A., et al. (2000) Immunity 13:255-264 ; ; Winau F., et al. (2004) Nat. Immunol. 5:169-174;

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol,1mM DTT

• Scientific Category :

  Immunology

• NCBI Accession Number :

  NP_001755

• Uniprot Accession Number :

  P29016

• Species :

  Human

• AA Sequence :

  SEHAFQ GPTSFHVIQT SSFTNSTWAQ TQGSGWLDDL QIHGWDSDSG TAIFLKPWSK GNFSDKEVAE LEEIFRVYIF GFAREVQDFA GDFQMKYPFE IQGIAGCELH SGGAIVSFLR GALGGLDFLS VKNASCVPSP EGGSRAQKFC ALIIQYQGIM ETVRILLYET CPRYLLGVLN AGKADLQRQV KPEAWLSSGP SPGPGRLQLV CHVSGFYPKP VWVMWMRGEQ EQQGTQLGDI LPNANWTWYL RATLDVADGE AAGLSCRVKH SSLEGQDIIL YWRNPTSIGS